Title: Mechanistic insights on the possible protective role of polyphenols extracted from Tamarixaphylla aerial parts against sodium arsenite-induced hepatotoxicity in rats

Abstract

Objective: Arsenic exposure is associated with the induction of hepatotoxicity. Current study was aimed to investigate the hepato-protective ability of polyphenolic components of Tamarixaphylla (TA) ethanolic extract against sodium arsenite (SA)-induced liver injury of rats. Methods & Results: Significantly, higher quantities of phenolic (318.7±2.5 mgg−1GAE) and flavonoid (250.69 ±3.3 mgg−1QE) contents were present. Inhibitory concentration (IC50) exhibited an excellent potential for antioxidant (IC50= 25.99 μg/mL) assay. High performance liquid chromatography (HPLC) confirmed the existence of myercetin (10.40ppm), sinapic acid (2.131ppm), kaempferol (0.486ppm), caffeic acid (5.094 ppm). Forty-two rats were divided into 7 groups. Group 1 received normal saline (2 mL/kg/day, orally for 21 days), Group 2 received SA (10mg/kg/day for 21 days), and Group 3 received SA alone for 7 days (10mg/kg) and continues with silymarine for 21 days (25mg/kg orally). Group 4, 5, 6 received SA alone for 7 days and continue with TA extract up to 21 days (125mg/kg, 250mg/kg, and 500mg/kg orally) respectively, and Group 7 received TA extract (500mg/kg) for 21 days. SA was administered to all treated groups for 21 days. Treatment with polyphenolic ethanolic extract of TA restored the hepatic indices and oxidative markers in a dose-dependent manner. The upregulation in tumor necrosis factor-α, interleukin-6, and cyclooxygenase-2 upon SA treatment suggesting inflammation was normalized by the treatment of rats. Above mentioned biochemical findings were supported well with histopathological screening.

Biography

Shaher Bano is a registered Pharmacist, Researcher and currently working as clinical research coordinator in research Centre of a trust hospital. After Pharmacy graduation in 2011, she started to practice my pharmacy profession from The Children‘s Hospital & the institute of child health, Lahore. Later on, she had served as retail/community pharmacist in a Private sector. Subsequently, having years of experience, she completed her M.Phil.