Title: Synthesis of azo-guanidine based molecules and their DNA interaction in alcoholic medium

Abstract

As DNA sensing has vast technology for researchers and other biotechnologists, we have introduced emerging class of azo and guanidine based molecules. We synthesized three new compounds (UA1, UA6, and UA7) and checked their potential application towards DNA sensing specially in alcoholic medium. These compounds were characterized in the solid and solution phase. These synthesized materials were characterized by elemental analysis, FTIF, UV-Visible, 1H NMR and 13C NMR spectroscopy. DNA interaction was further investigated by cyclic voltammetry and hydrodynamic studies. The results showed that compounds have moderate DNA binding properties such as binding constants are ranged 7.2×103, 2.4×103, 0.2×103 M-1, for UA1, UA6 and UA7 respectively. Upon binding with DNA, there was change of colour in solution can be easily seen with naked eye. This change in colour is due to blue shift in λmax value. These results indicated the potential of synthesized compounds as DNA sensors with detection limit 1.8, 5.8 and 4.0 ngµl-1 for UA1, UA6 and UA7 respectively. It is concluded that the binding mode is electrostatic and binding strength is moderate and is less than the other existing DNA sensing molecules. The good changes in wavelength indicated that better DNA sensors specially in ethanolic medium.

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