Background: Osteoporosis is a common disease characterized by low bone mass that can harm health. Lactoferrin has anti-microbial and plays a role in iron absorption, increase Osteogenesis and increase the immune responses. One of the goals of nanotechnology is mounting the drug on nanoparticle carriers and send them into the target cells. Materials and methods: In this study, lactoferrin (LF) of Camel milk were isolated using ion exchange chromatography and the purity and molecular weight was confirmed by SDS-PAGE electrophoresis and TBM test. The extracted LF has been purified by the аpplіcаtіon of HPLC and SDS-PAGE techniques. The concentrations of 25 and 100 µg/mL of LF were uploaded on perflourooctylbromide –NEPs(PFOB-NEPs). The interactions between LF and PFOB, LF and Alg have been studied by multiple spectroscopic as well as the zeta potential, and DLS pаrticlе size distribution. Results have displayed the quenched emissions of LF upon their interactions with PFOB and Alg.The effects of LF and LF were uploaded on PFOB-NEPs and Alg on growth of osteoblasts Cell Line (MG-63) was studied using MTT assay. Results: The average particle size before and after LF loading on PFOB did not change. Also, the zeta potential of nanoparticles is from -18.43 (without LF) has changed to +21.61 (LF 100 µg/ml), which confirms the loading of LF into PFOB-NEPs.The zeta potential value of LF has faced a decrease through the formation of LF– Alg complex. The best loading in both concentrations has occurred at the pH = 8. The results were shown, LF and LF were uploaded on PFOB-NEPs and Alg-NEPs increase Osteoblast cells proliferation significantly and There aren’t a significant difference between LF and LF were uploaded on PFOB- NEPs and there are a significant difference between LF and LF were uploaded on Alg-NEPs in both concentrations. The increase Osteoblast cells proliferation under the influence of different concentrations of LF uploaded on PFOB and Alg significant differences were shown.
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